Tesmin^em1Qsh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tesmin^em1Qsh
• Name: testis expressed metallothionein like; endonuclease-mediated mutation 1, Qinghua Shi
• MGI ID: MGI:7276184
• Synonyms: Mtl5^-
• Gene: Tesmin Location: Chr19:3438857-3457823 bp, + strand Genetic Position: Chr19, 3.15 cM, cytoband B
• Alliance: Tesmin^em1Qsh page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 19 bp (AGAGGAGGCCTCCAGCTGC) deletion in exon 2. Western blot analysis confirmed the absence of protein expression in the testes of homozygous mutant males. (J:324057)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tesmin Mutation: 23 strains or lines available

References

• Original: J:324057 Zhang X, et al., Nuclear translocation of MTL5 from cytoplasm requires its direct interaction with LIN9 and is essential for male meiosis and fertility. PLoS Genet. 2021 Aug;17(8):e1009753
• All: 1 reference(s)