Gt(ROSA)26Sor^{em1(CAG-APPIbe)Jjan}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-APPIbe)Jjan}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Joanna L Jankowsky
• MGI ID: MGI:7277689
• Synonyms: Gt(ROSA)26Sor^{em1(CAG-APPSweIbe)Jjan}, R26-APP^Ibe
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-APPIbe)Jjan} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:324255
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em1(CAG-APPIbe)Jjan} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

  expresses the Iberian (I716F) mutation

• Mutation details: The targeting vector is designed using a modified Ai3 (Gt(ROSA)26So^{tm3(CAG-EYFP)Hze)} vector with (from 5' to 3') a splice acceptor, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP (with stop codons in all 3 reading frames and a triple polyA signal) cassette, human APP sequence containing the Iberian (I716F) mutation, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), polyA sequence followed by an attB/attP flanked PGK-neo -PGKpA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The original report in Dis Model Mech 2022 indicated that both the Swedish and Iberian mutations were present in the allele, the allele has since been determined to carry only the Iberian mutation. In addition, an unintended mutation in the APP transmembrane domain, M647T by APP695 numbering (M722T by APP770 numbering) is present. This is not a known pathogenic mutation. (J:324255)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:324255 Koller EJ, et al., Temporal and spatially controlled APP transgene expression using Cre-dependent alleles. Dis Model Mech. 2022 May 1;15(5):dmm049330
• All: 1 reference(s)