Gt(ROSA)26Sor^{tm10.1(CAG-birA*,-mKate2)Amc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm10.1(CAG-birA*,-mKate2)Amc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 10.1, Andrew P McMahon
• MGI ID: MGI:7279067
• Synonyms: R26 BirA*G3-ER
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm10.1(CAG-birA*,-mKate2)Amc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:363524
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J/J

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed with, from 5' to 3', a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a floxed cassette containing a green fluorescent protein (GFP) sequence and a polyadenylation signal, a myc-tagged endoplasmic reticulum-directed promiscuous biotin ligase sequence (BirA*G3-ER), an internal ribosome entry site (IRES), and an mKate2 red fluorescent protein sequence. This targeting vector, which also includes an attB-neoR-attP cassette, was inserted into the Gt(ROSA)26Sor gene. PhiC31o-mediated recombination removed the neo cassette. (J:101977, J:363524)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:363524 Yang R, et al., A genetic model for in vivo proximity labelling of the mammalian secretome. Open Biol. 2022 Aug;12(8):220149
• All: 2 reference(s)