Tmprss12^em1Huizh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tmprss12^em1Huizh
• Name: transmembrane (C-terminal) protease, serine 12; endonuclease-mediated mutation 1, Hui Zhu
• MGI ID: MGI:7280668
• Synonyms: Tmprss12^-
• Gene: Tmprss12 Location: Chr15:100178743-100190943 bp, + strand Genetic Position: Chr15, 56.24 cM
• Alliance: Tmprss12^em1Huizh page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 2 bp (TA) deletion in exon 2. Western blot and immunofluorescence analysis confirmed the absence of protein in testes and sperm. (J:324488)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmprss12 Mutation: 30 strains or lines available

References

• Original: J:324488 Zhang J, et al., TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice. Front Cell Dev Biol. 2022;10:757042
• All: 1 reference(s)