Gata4^em1Vigr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gata4^em1Vigr
• Name: GATA binding protein 4; endonuclease-mediated mutation 1, Robert S Viger
• MGI ID: MGI:7280955
• Synonyms: GATA4 S261A
• Gene: Gata4 Location: Chr14:63436371-63509141 bp, - strand Genetic Position: Chr14, 33.24 cM
• Alliance: Gata4^em1Vigr page

Mutation origin

• Strain of Origin: (C57BL/6J x C3H/HeJ)F1/J

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Using an sgRNA and an ssODN template (gctcccaagggactatcctggcctagccctgctaaacacaaaacacttcctttctatgttgtagGcGgcttcccgccgggtaggcctctcctgtgccaactgccagactaccaccaccacg) with CRISPR/Cas9 technology, serine codon 261 (TCC) was changed to alanine (GCG) (p.S261A). This mutation blocks the phosphorylation of the residue. (J:308912)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gata4 Mutation: 36 strains or lines available

References

• Original: J:308912 Bergeron F, et al., Phosphorylation of GATA4 serine 105 but not serine 261 is required for testosterone production in the male mouse. Andrology. 2019 May;7(3):357-372
• All: 1 reference(s)