Sdhb^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Sdhb^{tm1c(EUCOMM)Hmgu}
• Name: succinate dehydrogenase complex, subunit B, iron sulfur (Ip); targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7282124
• Synonyms: SDHB^F
• Gene: Sdhb Location: Chr4:140688582-140706509 bp, + strand Genetic Position: Chr4, 73.24 cM, cytoband E1
• Alliance: Sdhb^{tm1c(EUCOMM)Hmgu} page
• IMPC: Sdhb gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:324842
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 140698021 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 140698743. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:324842)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sdhb Mutation: 29 strains or lines available

References

• Original: J:324842 Armstrong N, et al., SDHB knockout and succinate accumulation are insufficient for tumorigenesis but dual SDHB/NF1 loss yields SDHx-like pheochromocytomas. Cell Rep. 2022 Mar 1;38(9):110453
• All: 4 reference(s)