Eomes^{em1(icre)Mhos}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Eomes^{em1(icre)Mhos}
• Name: eomesodermin; endonuclease-mediated mutation 1, Mikio Hoshino
• MGI ID: MGI:7282233
• Synonyms: Eomes^{em1(icre)Inot}, Tbr2-T2A-iCre
• Gene: Eomes Location: Chr9:118307259-118315176 bp, + strand Genetic Position: Chr9, 70.21 cM, cytoband F3
• Alliance: Eomes^{em1(icre)Mhos} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Eomes^{em1(icre)Mhos} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Eomes (MGI:1201683)

• Mutation details: Using CRISPR/cas9 technology, a T2A-iCre expression cassette was inserted into Eomes (Tbr2) gene just upstream of the stop codon. (J:314171)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Eomes (mouse) Summary of all recombinase alleles driven by Eomes. Alliance mouse Eomes gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eomes Mutation: 44 strains or lines available

References

• Original: J:314171 Inoue YU, et al., An Optimized Preparation Method for Long ssDNA Donors to Facilitate Quick Knock-In Mouse Generation. 7 new recombinases (including the one already entered), and 5 different reporter alleles. Cells. 2021 Apr 30;10(5)
• All: 1 reference(s)