Gt(ROSA)26Sor^{tm1(CAG-EGFP,-BphP1/mCherry/TetR)Vvv}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-EGFP,-BphP1/mCherry/TetR)Vvv}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Vladislav Verkhusha
• MGI ID: MGI:7284375
• Synonyms: loxP-BphP1-mCherry-TetR
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-EGFP,-BphP1/mCherry/TetR)Vvv} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:325201
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: BALB/c

Mutation description

• Allele Type: Targeted (Inducible, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxP site, an enhanced green fluorescent protein sequence followed by an SV40 polyA signal, a loxP site, the RpBphP1/mCherry/TetR fusion protein (containing the sequences encoding a purple photosynthetic bacterium Rhodopseudomonas palustris-derived bacteriophytochrome photoreceptor P1 [RpBphP1], the monomeric red fluorescent protein [mCherry] and the tet repressor sequence [TetR]), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA sequence (BGHpA) and an FRT-flanked neomycin resistance cassette. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus via electroporation. (J:325201)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

Notes

ES cells = iTLb

References

• Original: J:325201 Kasatkina LA, et al., Optogenetic manipulation and photoacoustic imaging using a near-infrared transgenic mouse model. Nat Commun. 2022 May 19;13(1):2813
• All: 1 reference(s)