Parm1^em1(cre)Ngai
Endonuclease-mediated Allele Detail

Summary

• Symbol: Parm1^em1(cre)Ngai
• Name: prostate androgen-regulated mucin-like protein 1; endonuclease-mediated mutation 1, John Ngai
• MGI ID: MGI:7286053
• Gene: Parm1 Location: Chr5:91665559-91771870 bp, + strand Genetic Position: Chr5, 44.78 cM
• Alliance: Parm1^em1(cre)Ngai page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Recombinase)
• Mutation: Insertion
• Parm1^em1(cre)Ngai expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Parm1 (MGI:2443349)

• Mutation details: Using CRISPR/Cas9 technology, a P2A-Cre (encoding a viral 2A oligopeptide that mediates ribosomal skipping and a codon-optimized cre gene) and a myc-tag were inserted into the targetted STOP codon of the Prostate Androgen-Regulated Mucin-Like Protein 1 gene (Parm1) on chromosome 5 in embryonic stem cells. Chimeric mice were generated by aggregating ES cells with morula-stage embryos and bred for germline transmission. (J:101977)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Parm1 (mouse) Summary of all recombinase alleles driven by Parm1. Alliance mouse Parm1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Parm1 Mutation: 23 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)