Gt(ROSA)26Sor^{tm1(CAG-Clomeleon*)Kjst}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Clomeleon*)Kjst}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Kevin J Staley
• MGI ID: MGI:7287847
• Synonyms: sCLM
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Clomeleon*)Kjst} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:316904
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAAG), a loxP-flanked STOP/Neo cassette, SuperClomeleon (an improved fluorescent fusion protein containing Cerulean and YFP) and a polyA sequence. SuperClomeleon differs from Clomeleon by the following additions: a short interdomain linker, an S30R point mutation in both domains, intermediate affinity-tuning mutations, Q69T/V163A double mutant in YFP and replacement of the CFP domain with Cerulean. It provides a higher choloride sensitivity than Clomeleon. (J:316904, J:325762)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:316904 Rahmati N, et al., Unique Actions of GABA Arising from Cytoplasmic Chloride Microdomains. J Neurosci. 2021 Jun 9;41(23):4957-4975
• All: 2 reference(s)