Serpina3c^em1Smoc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Serpina3c^em1Smoc
• Name: serine (or cysteine) peptidase inhibitor, clade A, member 3C; endonuclease-mediated mutation 1, Shanghai Model Organisms Center
• MGI ID: MGI:7292227
• Synonyms: SERPINA3C-KO
• Gene: Serpina3c Location: Chr12:104113166-104120187 bp, - strand Genetic Position: Chr12, 53.46 cM, cytoband F1
• Alliance: Serpina3c^em1Smoc page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology was used to delete exons 2-5 of the mouse Serpina3c gene by non-homologous recombination. qPCR analysis demonstrated that mRNA expression was abolished in multiple tissues, including brown adipose tissue (BAT), inguinal and epididymal white adipose tissue (WAT), liver, and muscle. Western blot analysis confirmed the absence of protein expression in the inguinal and epididymal WAT of homozygous mutant mice. (J:238766)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Serpina3c Mutation: 32 strains or lines available

References

• Original: J:238766 Shanghai Model Organisms Center, Information obtained from the Shanghai Model Organisms Center (SMOC), Shanghai, China. MGI Direct Data Submission. 2017
• All: 2 reference(s)