Spem2^em1Smoc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Spem2^em1Smoc
• Name: SPEM family member 2; endonuclease-mediated mutation 1, Shanghai Model Organisms Center
• MGI ID: MGI:7292552
• Synonyms: Spem2^-
• Gene: Spem2 Location: Chr11:69707392-69709291 bp, - strand Genetic Position: Chr11, 42.89 cM
• Alliance: Spem2^em1Smoc page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The CRISPR/Cas9 genome editing system was used to target the entire Spem2 gene, including the 32-bp-long 5-untranscribed region, exons 1-3 (except for the last 42 bp of exon 3), and 2 introns in between. A 1894-bp deletion was confirmed by direct sequencing analysis. RT-PCR analysis demonstrated the absence of expression in the testes of homozygous mutant males. (J:238766, J:354200)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Spem2 Mutation: 15 strains or lines available

References

• Original: J:238766 Shanghai Model Organisms Center, Information obtained from the Shanghai Model Organisms Center (SMOC), Shanghai, China. MGI Direct Data Submission. 2017
• All: 2 reference(s)