Sox30^em1Laye
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sox30^em1Laye
• Name: SRY (sex determining region Y)-box 30; endonuclease-mediated mutation 1, Lan Ye
• MGI ID: MGI:7310078
• Synonyms: Sox30^-
• Gene: Sox30 Location: Chr11:45871137-45908821 bp, + strand Genetic Position: Chr11, 27.49 cM
• Alliance: Sox30^em1Laye page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 47-bp deletion in exon 2 within the HMG Box domain. This deletion produces a premature termination codon. RT-PCR and Western blot analysis confirmed the absence of full-length transcript and protein, respectively, in testis lysates from adult homozygous mutant mice. (J:263980)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox30 Mutation: 19 strains or lines available

References

• Original: J:263980 Bai S, et al., Sox30 initiates transcription of haploid genes during late meiosis and spermiogenesis in mouse testes. Development. 2018 Jul 4;145(13):dev164855
• All: 1 reference(s)