Rpap3^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rpap3^{tm1c(KOMP)Wtsi}
• Name: RNA polymerase II associated protein 3; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:7310425
• Synonyms: Rpap3^flox
• Gene: Rpap3 Location: Chr15:97572978-97603706 bp, - strand Genetic Position: Chr15, 53.54 cM, cytoband F2
• Alliance: Rpap3^{tm1c(KOMP)Wtsi} page
• IMPC: Rpap3 gene page

Mutation origin

• Mutant Cell Line: EPD0303_1_H10
• Germline Transmission: Earliest citation of germline transmission: J:314346
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 97590808 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 97591624. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:314346)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rpap3 Mutation: 34 strains or lines available

References

• Original: J:314346 Maurizy C, et al., The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium. Nat Commun. 2021 Aug 10;12(1):4810
• All: 1 reference(s)