Del(6Spam1-Hyal5)1Eki
Endonuclease-mediated Allele Detail

Summary

• Symbol: Del(6Spam1-Hyal5)1Eki
• Name: deletion, Chr 6, Ekyune Kim 1
• MGI ID: MGI:7310454
• Synonyms: Spam1^-/Hyal5^-
• Gene: Del(6Spam1-Hyal5)1Eki Location: unknown Genetic Position: Chr6, Syntenic

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intergenic deletion
• Del(6Spam1-Hyal5)1Eki involves 2 genes/genome features (Spam1, Hyal5) View all
• Mutation details: CRISPR/Cas9 technology deleted a 56.9 kb genomic region encompassing Spam1 and Hyal5. Western blot analysis demonstrated absence of both proteins in cauda epididymal sperm. In addition, zymographic assays confirmed the complete absence of hyaluronidase activity in sperm. (J:294353)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(6Spam1-Hyal5)1Eki Mutation: 0 strains or lines available

References

• Original: J:294353 Park S, et al., SPAM1/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus-oocyte complex penetration defect. FASEB J. 2019 Dec;33(12):14440-14449
• All: 1 reference(s)