Prl8a2^em1(icre)Fjd
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prl8a2^em1(icre)Fjd
• Name: prolactin family 8, subfamily a, member 2; endonuclease-mediated mutation 1, Franco J DeMayo
• MGI ID: MGI:7311604
• Synonyms: Prl8a2iCre
• Gene: Prl8a2 Location: Chr13:27529656-27538199 bp, + strand Genetic Position: Chr13, 12.41 cM
• Alliance: Prl8a2^em1(icre)Fjd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:326357
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Prl8a2^em1(icre)Fjd expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Prl8a2 (MGI:894281)

• Mutation details: Using CRISPR/Cas9 endonuclease-mediated genome editing, a donor plasmid designed to insert a codon improved cre recombinase (iCre) and a polyadenylation sequence immediately downstream of the start codon of the prolactin family 8, subfamily a, member 2 (Prl8a2) gene, a single guide RNA (ACTAGCAGTCTTATCCAACCNGG), and the cas9 nuclease with a puromycin resistance sequence were transfected into G4 embryonic stem (ES) cells. (J:326357)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Prl8a2 (mouse) Summary of all recombinase alleles driven by Prl8a2. Alliance mouse Prl8a2 gene page

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prl8a2 Mutation: 20 strains or lines available

References

• Original: J:326357 Dickson MJ, et al., Inserting Cre recombinase into the Prolactin 8a2 gene for decidua-specific recombination in mice. Genesis. 2022 May;60(4-5):e23473
• All: 2 reference(s)