Adgb^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Adgb^{tm1d(KOMP)Wtsi}
• Name: androglobin; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7311625
• Gene: Adgb Location: Chr10:10211453-10348070 bp, - strand Genetic Position: Chr10, 3.44 cM
• Alliance: Adgb^{tm1d(KOMP)Wtsi} page
• IMPC: Adgb gene page

Mutation origin

• Mutant Cell Line: EPD0707_3_H06
• Germline Transmission: Earliest citation of germline transmission: J:326350
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 10282651 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 10283721. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:326350)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adgb Mutation: 89 strains or lines available

References

• Original: J:326350 Keppner A, et al., Androglobin, a chimeric mammalian globin, is required for male fertility. Elife. 2022 Jun 14;11:e72374
• All: 1 reference(s)