Rfx2^tm1Hncs
Targeted Allele Detail

Summary

• Symbol: Rfx2^tm1Hncs
• Name: regulatory factor X, 2 (influences HLA class II expression); targeted mutation 1, Chunsheng Han
• MGI ID: MGI:7312142
• Synonyms: Rfx2^-
• Gene: Rfx2 Location: Chr17:57082897-57138013 bp, - strand Genetic Position: Chr17, 29.5 cM, cytoband D
• Alliance: Rfx2^tm1Hncs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:230575
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 6 and 7, encoding the DNA binding domain, were replaced with a phosphoglycerate kinase-neomycin-resistance cassette using homologous recombination. RT-PCR analysis showed that deletion of exon 6 and 7 results in the splicing of exon 5 to exon 8, leading to a frame shift and premature termination at an out-of-frame stop codon. Western blot analysis confirmed the absence of protein expression in total testis extracts from homozygous mutant mice. (J:230575)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rfx2 Mutation: 79 strains or lines available

References

• Original: J:230575 Wu Y, et al., Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis. Sci Rep. 2016;6:20435
• All: 1 reference(s)