Asb17^em1Bzhg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Asb17^em1Bzhg
• Name: ankyrin repeat and SOCS box-containing 17; endonuclease-mediated mutation 1, Bo Zheng
• MGI ID: MGI:7314167
• Synonyms: Asb17-KO
• Gene: Asb17 Location: Chr3:153549884-153559252 bp, + strand Genetic Position: Chr3, 78.68 cM
• Alliance: Asb17^em1Bzhg page

Mutation origin

• Strain of Origin: CD-1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9-based gene editing generated an 8-bp deletion in exon 1, as confirmed by PCR amplification and further Sanger sequencing. Immunofluorescence staining analysis confirmed the absence of protein expression in homozygous mutant testes. (J:324157)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Asb17 Mutation: 17 strains or lines available

References

• Original: J:324157 Shen C, et al., E3 ubiquitin ligase ASB17 is required for spermiation in mice. Transl Androl Urol. 2021 Dec;10(12):4320-4332
• All: 1 reference(s)