Misfadt^em1Psv
Endonuclease-mediated Allele Detail

Summary

• Symbol: Misfadt^em1Psv
• Name: mitochondrial sheath formation associated divergent transcript; endonuclease-mediated mutation 1, Petr Svoboda
• MGI ID: MGI:7330166
• Synonyms: Sirena1^-
• Gene: Misfadt Location: Chr7:46617458-46625659 bp, - strand Genetic Position: Chr7, 30.65 cM
• Alliance: Misfadt^em1Psv page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 1678 bp deletion including the promoter, exon 1 and exon 2 (7:46974556-46976233). qPCR and in situ hybridization confirmed absence of transcript in oocytes. (J:291605)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Misfadt Mutation: 0 strains or lines available

References

• Original: J:291605 Ganesh S, et al., The most abundant maternal lncRNA Sirena1 acts post-transcriptionally and impacts mitochondrial distribution. Nucleic Acids Res. 2020 Apr 6;48(6):3211-3227
• All: 1 reference(s)