Cmtm5^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cmtm5^{tm1d(KOMP)Wtsi}
• Name: CKLF-like MARVEL transmembrane domain containing 5; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7330369
• Synonyms: Cmtm5^-
• Gene: Cmtm5 Location: Chr14:55173927-55176734 bp, + strand Genetic Position: Chr14, 28.0 cM, cytoband C1
• Alliance: Cmtm5^{tm1d(KOMP)Wtsi} page
• IMPC: Cmtm5 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:327261
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 55174959 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 55176866. The critical exon(s) is/are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene. Cre-mediated recombination in the germline deleted the floxed exon(s). (J:327261)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cmtm5 Mutation: 17 strains or lines available

References

• Original: J:327261 Buscham TJ, et al., Progressive axonopathy when oligodendrocytes lack the myelin protein CMTM5. Elife. 2022 Mar 11;11:e75523
• All: 1 reference(s)