Igs7^{tm215(CAG-ACR2*,CAG-GCaMP7s)Tasic}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm215(CAG-ACR2*,CAG-GCaMP7s)Tasic}
• Name: intergenic site 7; targeted mutation 215, Bosiljka Tasic
• MGI ID: MGI:7331591
• Synonyms: Ai215, Ai215^{TICL-stGtACR2-HA-ICL-GCaMP7s-SS}, Igs7^{tm215(CAG-ACR2*,CAG-GCaMP7s)Hze}
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm215(CAG-ACR2*,CAG-GCaMP7s)Tasic} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector contains (from 5' to 3') a Bxb1 attL site, a partial green fluorescent protein sequence (non-functional 500bp fragment to increase the space between the Bxb1 attB site and the insulator sequence to improve stability of the targeting vector), two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from pCAGGS), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), the coding sequence of stGtACR2 (GtACR2 with targeting sequence TS and Kv2.1 and HA tag sequence), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, the CAG promoter, a lox2272-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-TKpA), the coding sequence of GCaMP7s (with GSG linker sequence and supersoma sequence), a BGH polyA, a PhiC31 attB site, a PGK-5'hygro cassette, an RNA splice donor, a Bxb1 attR site, SA, 3'hygro, SV40pA, and PhiC31 attP. GtACR2 is derived from the Guillardia theta anion channelrhodopsin 2 gene. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 70 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)