Trip6^tm1.2Pher
Targeted Allele Detail

Summary

• Symbol: Trip6^tm1.2Pher
• Name: thyroid hormone receptor interactor 6; targeted mutation 1.2, Peter Herrlich
• MGI ID: MGI:7331652
• Gene: Trip6 Location: Chr5:137308160-137312469 bp, - strand Genetic Position: Chr5, 76.34 cM
• Alliance: Trip6^tm1.2Pher page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:312425
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2 and a loxP flanked neomycin resistance cassette was inserted downstream of exon 9. The neomycin resistance cassette was deleted via electroporation of ES cells with a cre expression plasmid. Exons 2 to 9 were deleted via cre-mediated recombination. RT-qPCR and immunoblotting confirmed absence of mRNA and protein. (J:312425)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trip6 Mutation: 18 strains or lines available

References

• Original: J:312425 Shukla S, et al., TRIP6 functions in brain ciliogenesis. Nat Commun. 2021 Oct 7;12(1):5887
• All: 1 reference(s)