Ccdc186^{Tn(pb-Act-RFP)1Xwu}
Transposon induced Allele Detail

Summary

• Symbol: Ccdc186^{Tn(pb-Act-RFP)1Xwu}
• Name: coiled-coil domain containing 186; transposon insertion 1, Xiaohui Wu
• MGI ID: MGI:7332832
• Synonyms: Otg1^PB
• Gene: Ccdc186 Location: Chr19:56775913-56810622 bp, - strand Genetic Position: Chr19, 51.99 cM
• Alliance: Ccdc186^{Tn(pb-Act-RFP)1Xwu} page

Mutation origin

• Strain of Origin: FVB/NJ

Mutation description

• Allele Type: Transposon induced (Null/knockout)
• Mutation: Transposon insertion
• Mutation details: A piggyBac (PB) transposon element derived from cabbage looper moth Trichoplusia ni is introduced in exon 8 of the gene. Its components were introduced by coinjection of mouse oocytes with circular piggyBac donor and helper plasmids. The donor plasmid contained the piggyBac transposon element in which the transposase sequence was replaced by a beta actin (Actb) promoter-driven red flourescent protein (RFP) reporter gene flanked by piggyBac-specific inverted terminal repeat sequences. The helper plasmid employs the Actb promoter to drive expression of a piggyBac transposase gene lacking the terminal inverted repeat sequences required for its transposition. The insertion contains the RFP expression unit flanked by transposon (donor) and transposase (helper) sequences. RFP is flanked by transposon donor and transposase helper sequences in the final construct. Western blot analysis indicates no expression of protein in various tissues including embryo, brain, lung, heart, stomach, liver, kidney, pancreas, and gut. (J:250070)

Expression

In Mice Carrying this Mutation:

22 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccdc186 Mutation: 36 strains or lines available

References

• Original: J:250070 Wang G, et al., Disruption of the Golgi protein Otg1 gene causes defective hormone secretion and aberrant glucose homeostasis in mice. Cell Biosci. 2016;6:41
• All: 1 reference(s)