Cyfip2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cyfip2^{tm1c(EUCOMM)Wtsi}
• Name: cytoplasmic FMR1 interacting protein 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7338923
• Synonyms: Cyfip2^flox
• Gene: Cyfip2 Location: Chr11:46084677-46203686 bp, - strand Genetic Position: Chr11, 27.61 cM, cytoband B1.2
• Alliance: Cyfip2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Cyfip2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:326379
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 46170548 of Chromosome 11 upstream of exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 46171429. Exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying the Cyfip2^{tm1a(EUCOMM)Wtsi} allele. (J:326379)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyfip2 Mutation: 59 strains or lines available

References

• Original: J:326379 Chaya T, et al., Deficiency of the neurodevelopmental disorder-associated gene Cyfip2 alters the retinal ganglion cell properties and visual acuity. Hum Mol Genet. 2022 Feb 21;31(4):535-547
• All: 3 reference(s)