Rr275^em1Mad
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr275^em1Mad
• Name: regulatory region 275; endonuclease-mediated mutation 1, Michael A Dyer
• MGI ID: MGI:7341365
• Synonyms: R2-22 delta
• Gene: Rr275 Location: Chr12:84592972-84597016 bp Genetic Position: Chr12, Syntenic
• Alliance: Rr275^em1Mad page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Intergenic deletion
• Mutation details: This Vsx2 enhancer was targeted with sgRNAs (UGGUGAUGGAGCGCAUAGCG and GGGCAGUGCAUCCCCUGUGG) and an ssODN template (CCAGCCACTGTCCCAGCCCGGGCTCTAAGTCTGACCCAAGCCGAAGTGCCTGCGCCCCGCTGGTGGGTGGGTGTGGACATCTTCCAGGCCCCAGGACACACCAGGAATCAAGAGGGAGGT) using CRISPR/Cas9 technology, resulting in a 4,045 bp deletion (chr12:84592972-84597016 GRCm39). (J:328714)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr275 Mutation: 0 strains or lines available

References

• Original: J:328714 Honnell V, et al., Identification of a modular super-enhancer in murine retinal development. Nat Commun. 2022 Jan 11;13(1):253
• All: 1 reference(s)