Gt(ROSA)26Sor^{tm10(CAG-Confetti2)Bzsh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm10(CAG-Confetti2)Bzsh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 10, Bin Zhou
• MGI ID: MGI:7341593
• Synonyms: R26-Confetti2, Rosa26-rox-Stop-rox-Confetti
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm10(CAG-Confetti2)Bzsh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278107
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: CAGroxStoproxConfetti was targeted to the Rosa26 gene locus with four fluorescent protein sequences uniquely positioned in a tandem fashion and delimited by loxP sites in the opposite orientations. Specifically, it is composed of two adjacent floxed head-to-tail tandem dimers. The first head-to-tail dimer contains a loxP site and mCFP in forward orientation and RFP in reverse orientation followed by a reverese loxP site. The second head-to-tail dimer contains a loxP site and YFP in forward orientation, and nGFP in reverse orientation followed by another reverse loxP site. The allele contains roxStoprox cassettes before confetti, so that only after dual Drerox and CreloxP recombination could single-color fluorescence reporters such as yellow fluorescent protein (YFP), green fluorescent protein (GFP) and red fluorescent protein (RFP) be expressed in Dre+Cre+ cells and their descendants. Without cre recombination, only mCerulean CFP is expressed after Dre-rox recombination. (J:278107)

Expression

In Mice Carrying this Mutation:

34 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 958 strains or lines available

References

• Original: J:278107 Liu Q, et al., Lung regeneration by multipotent stem cells residing at the bronchioalveolar-duct junction. Nat Genet. 2019 Apr;51(4):728-738
• All: 4 reference(s)