Rr278^em1Flc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr278^em1Flc
• Name: regulatory region 278; endonuclease-mediated mutation 1, Frank Conlon
• MGI ID: MGI:7341598
• Synonyms: Myh11^deltai3
• Gene: Rr278 Location: Chr16:14083451-14083463 bp Genetic Position: Chr16, Syntenic
• Alliance: Rr278^em1Flc page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Intragenic deletion
• Mutation details: This CHD4/GATA4/NKX2-5/TBX5-binding site-containing 414 bp cis-regulatory element (CRE) in Myh11 intron 3, suppressing Myh11 expression, was targeted with sgRNAs using CRISPR/Cas9 technology, resulting in the deletion of the GATA-binding region all four transcription factors bind to. (J:324708)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr278 Mutation: 0 strains or lines available

References

• Original: J:324708 Robbe ZL, et al., CHD4 is recruited by GATA4 and NKX2-5 to repress noncardiac gene programs in the developing heart. Genes Dev. 2022 Apr 1;36(7-8):468-482
• All: 1 reference(s)