Cryaa^{em1(cre/ERT2)Xfan}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cryaa^{em1(cre/ERT2)Xfan}
• Name: crystallin, alpha A; endonuclease-mediated mutation 1, Xingjun Fan
• MGI ID: MGI:7345512
• Synonyms: Cryaa(2A-Cre-ERT2), Cryaa^{tm1(cre/ERT2)Xfan}
• Gene: Cryaa Location: Chr17:31896905-31900704 bp, + strand Genetic Position: Chr17, 17.09 cM, cytoband A3-B
• Alliance: Cryaa^{em1(cre/ERT2)Xfan} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Cryaa^{em1(cre/ERT2)Xfan} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cryaa (MGI:88515)

• Mutation details: CRISPR/cas9 mediated recombination using guide RNAs inserted a P2A-CreERT2 cassette creating an in frame fusion to the C-terminal portion of the gene. (J:101977, J:348650)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cryaa (mouse) Summary of all recombinase alleles driven by Cryaa. Alliance mouse Cryaa gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cryaa Mutation: 15 strains or lines available

References

• Original: J:348650 Wei Z, et al., A tamoxifen-inducible Cre knock-in mouse for lens-specific gene manipulation. Exp Eye Res. 2022 Nov 11;226:109306
• All: 2 reference(s)