Fmn1em1Zllr
Endonuclease-mediated Allele Detail
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Symbol: |
Fmn1em1Zllr |
Name: |
formin 1; endonuclease-mediated mutation 1, Rolf Zeller |
MGI ID: |
MGI:7346402 |
Synonyms: |
EC1delta |
Gene: |
Fmn1 Location: Chr2:113158081-113547112 bp, + strand Genetic Position: Chr2, 57.3 cM, cytoband C1-qter
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Alliance: |
Fmn1em1Zllr page
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Strain of Origin: |
Not Applicable
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Allele Type: |
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Endonuclease-mediated (Modified regulatory region, Null/knockout) |
Mutation: |
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Intragenic deletion
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Fmn1em1Zllr involves 3 genes/genome features (Rr285, Rr284, Rr26)
View all
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Mutation details: An enhancer cluster containing Grem1 limb regulatory regions Rr285 (CRM2), Rr284 (CRM3) and Rr26 (CRM4), located in Fmn1 introns 15 and 13 [in reference to ENSMUST00000081349], was targeted with sgRNAs (targeting CACCGTGGCTTACCAGACTAGCGGT and CACCGAATGGTCTGATCGCCA) using CRISPR/Cas9 technology, resulting in a 71.2 kb deletion (chr2:113467121-113538319 GRCm39) from intron 13 to 16 (so including exons 14-16).
(J:312378)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Fmn1 Mutation: |
76 strains or lines available
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Original: |
J:312378 Malkmus J, et al., Spatial regulation by multiple Gremlin1 enhancers provides digit development with cis-regulatory robustness and evolutionary plasticity. Nat Commun. 2021 Sep 21;12(1):5557 |
All: |
1 reference(s) |
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