Sox2ot^em1Dyd
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sox2ot^em1Dyd
• Name: SOX2 overlapping transcript (non-protein coding); endonuclease-mediated mutation 1, Dao-Yin Dong
• MGI ID: MGI:7365204
• Synonyms: Sox2ot promoter-deleted
• Gene: Sox2ot Location: Chr3:34614530-34732142 bp, + strand Genetic Position: Chr3, 16.56 cM
• Alliance: Sox2ot^em1Dyd page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Intragenic deletion
• Mutation details: The Sox2ot downstream promoter was targeted with an sgRNA using CRISPR/Cas9 technology, resulting in a deletion of the promoter (Rr297) and exons 1 and 2. Transcripts containing exons 3-5 were still expressed in E10.5 embryos, possibly from the upstream promoter. (J:310878)

Expression

In Mice Carrying this Mutation:

15 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox2ot Mutation: 1 strain or line available

References

• Original: J:310878 Li PY, et al., CRISPR/Cas9-mediated gene editing on Sox2ot promoter leads to its truncated expression and does not influence neural tube closure and embryonic development in mice. Biochem Biophys Res Commun. 2021 Oct 8;573:107-111
• All: 1 reference(s)