Sepsecs^tm1Usch
Targeted Allele Detail

Summary

• Symbol: Sepsecs^tm1Usch
• Name: Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase; targeted mutation 1, Ulrich Schweizer
• MGI ID: MGI:7366908
• Synonyms: Sepsecs^Y334C
• Gene: Sepsecs Location: Chr5:52797429-52827050 bp, - strand Genetic Position: Chr5, 28.44 cM
• Alliance: Sepsecs^tm1Usch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:315052
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Single point mutation
• Mutation details: The targeting construct introduced the mutation responsible for the amino acid substitution of tyrosine to cysteine at position 334 (Y334C). Two silent mutations (p.I324 and p.T325) were also introduced. An FRT-flanked selection cassette is present in intron 8. (J:315052)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sepsecs Mutation: 25 strains or lines available

References

• Original: J:315052 Fradejas-Villar N, et al., Missense mutation in selenocysteine synthase causes cardio-respiratory failure and perinatal death in mice which can be compensated by selenium-independent GPX4. Redox Biol. 2021 Nov 12;48:102188
• All: 1 reference(s)