Isl1^em1Vave
Endonuclease-mediated Allele Detail

Summary

• Symbol: Isl1^em1Vave
• Name: ISL1 transcription factor, LIM/homeodomain; endonuclease-mediated mutation 1, Vasanth Vedantham
• MGI ID: MGI:7367280
• Synonyms: Isl1^3xFLAG
• Gene: Isl1 Location: Chr13:116434817-116446225 bp, - strand Genetic Position: Chr13, 64.87 cM, cytoband D2.3
• Alliance: Isl1^em1Vave page

Mutation origin

• Strain of Origin: FVB/NJ

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: Sequence coding for three FLAG tags was inserted in-frame immediately upstream of the stop codon using sgRNAs (targeting CATGGTAGCCAGTCCTATTG and TCCTCATGCCTCAATAGGAC) and an ssODN template (ATCGATGTCCTCGCAGCTCCCAGATACACCCAACAGCATGGTAGCCAGTCCTATTGAAGCAGGTGGTGGTGGTTCTGACTACAAAGACCATGACGGTGATTATAAAGATCATGATATCGATTACAAGGATGACGATGACAAGTGAGGAACATTCATT) using CRISPR/Cas9 technology. (J:308878)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Isl1 Mutation: 35 strains or lines available

References

• Original: J:308878 Galang G, et al., ATAC-Seq Reveals an Isl1 Enhancer That Regulates Sinoatrial Node Development and Function. Circ Res. 2020 Dec 4;127(12):1502-1518
• All: 1 reference(s)