H2-K1^b-em1Tyj
Endonuclease-mediated Allele Detail

Summary

• Symbol: H2-K1^b-em1Tyj
• Name: histocompatibility 2, K1, K region; endonuclease-mediated mutation 1, Tyler Jacks
• MGI ID: MGI:7382781
• Synonyms: H2-K1^em1Tyj, K^bStrep
• Gene: H2-K1 Location: Chr17:34214986-34219316 bp, - strand Genetic Position: Chr17, 17.98 cM
• Alliance: H2-K1^b-em1Tyj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:330295
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (B6.129P2-Trp53^tm1Brn/J x B6(129X1)-Dst^em13Dcr/DcrJ)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 genome editing technology is used to insert a cre-inducible affinity tag, StrepTagII, into intron 1 of the gene. The vector included two pairs of inward-facing heterospecific Lox sites (loxP and lox2272) flanking an inverted StrepTagII. (J:330295)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any H2-K1 Mutation: 60 strains or lines available

Notes

ES cells = KP*1 created by crossing B6.129P2-Trp53/J and B6(129X1)-Dst/DcrJ

References

• Original: J:330295 Jaeger AM, et al., Deciphering the immunopeptidome in vivo reveals new tumour antigens. Nature. 2022 Jul;607(7917):149-155
• All: 1 reference(s)