Col1a1^{tm25(tetO-cre/PhoCl/ERT2,CAG-rtTA)Nki}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm25(tetO-cre/PhoCl/ERT2,CAG-rtTA)Nki}
• Name: collagen, type I, alpha 1; targeted mutation 25, The Netherlands Cancer Institute
• MGI ID: MGI:7382867
• Synonyms: Col1a1^{tm1(tetO-cre/ERT2,CAG-rtTA)Jvr}, DiLiCre2.0
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm25(tetO-cre/PhoCl/ERT2,CAG-rtTA)Nki} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:330674
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, RMCE-ready, Recombinase, Transactivator)
• Inducer: tamoxifen, doxycycline/tetracycline, and light
• Mutation: Insertion
• Col1a1^{tm25(tetO-cre/PhoCl/ERT2,CAG-rtTA)Nki} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: RMCE retargeted Col1a1^tm22Nki, to replace the F3/frt flanked PGK-Puro casette in the 3' UTR of Cola1a by a "RMCE" plasmid encoding F3-TRE3GV-Cre-PhoCl-2xERT2-CAG-rtTA(Tet-On 3G)-frt , by cotransfecting a Flpe-expressing plasmid to facilitate recombination into the 3' UTR of the Col1a1 locus. The transgene is a F3/FRT flanked hybrid gene, consisting of a Tet-responsive promoter that can be activated by binding of Tet-On3G, a Cre recombinase, a photocleavable protein (PhoCl) that dissociates into two fragments after light-induced cleavage and 2xERT2. It is followed by the CAG promoter which drives the expression of a modified artificial transcription factor (Tet-On3G) that binds to and activates promoters containing the tet operator in the presence of doxycycline. This resulted in a doxycycline- and light inducible Cre recombinase (DiLiCre2.0) mouse line. When treated with doxycycline the photoconvertible fluorescent protein (PhoCl) emits a green light, upon 405nm light illumination the fluorescent protein switches from green to an unstable red fluorescent protein which cleaves within minutes, releasing Cre from the ER and allowing it to translocate to the nucleus. The protein will also translocate intact (including its Cre recombinase activity) from the ER to the nucleus upon treatment with tamoxifen. (J:330674)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

References

• Original: J:330674 Vizoso M, et al., A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing. Nat Commun. 2022 Oct 28;13(1):6442
• All: 1 reference(s)