Lyset^em1Janc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Lyset^em1Janc
• Name: lysosomal enzyme trafficking factor; endonuclease-mediated mutation 1, Jan Carette
• MGI ID: MGI:7384326
• Synonyms: delta184
• Gene: Lyset Location: Chr12:102710021-102713820 bp, + strand Genetic Position: Chr12, 52.15 cM
• Alliance: Lyset^em1Janc page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology targeting exon 2 generated a 184 bp deletion resulting in a frameshift. Western blot analysis confirmed absence of protein. (J:330704)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lyset Mutation: 5 strains or lines available

References

• Original: J:330704 Richards CM, et al., The human disease gene LYSET is essential for lysosomal enzyme transport and viral infection. Science. 2022 Sep 8;:eabn5648
• All: 1 reference(s)