Guk1^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Guk1^em1(IMPC)J
• Name: guanylate kinase 1; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:7385089
• Gene: Guk1 Location: Chr11:59074701-59083069 bp, - strand Genetic Position: Chr11, 37.05 cM
• Alliance: Guk1^em1(IMPC)J page
• IMPC: Guk1 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences AGCATAGCTGATAGGCTCCA and CATAGCAGTCCTGTGTAGGG, which resulted in a 429 bp deletion beginning at Chromosome 11 position 59,185,683 bp and ending after 59,186,111 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000104732 (exon 4) and 332 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 72 and early truncation 10 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Guk1 Mutation: 13 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 2 reference(s)