Cep72^em1Lmgg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cep72^em1Lmgg
• Name: centrosomal protein 72; endonuclease-mediated mutation 1, Mengcheng Luo
• MGI ID: MGI:7398570
• Synonyms: Cep72 KO
• Gene: Cep72 Location: Chr13:74184619-74210418 bp, - strand Genetic Position: Chr13, 40.15 cM, cytoband C1
• Alliance: Cep72^em1Lmgg page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 gene-edited technology deleted 47 bp in exon 3 as confirmed by Sanger sequencing. The deletion resulted in an open reading frameshift and generated a premature termination codon. Western blot analysis demonstrated the complete absence of protein expression in the testis of homozygous mutant mice. (J:330932)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cep72 Mutation: 27 strains or lines available

References

• Original: J:330932 Chen Z, et al., Loss of Cep72 affects the morphology of spermatozoa in mice. Front Physiol. 2022;13:948965
• All: 1 reference(s)