Wapl^tm1.1Kpfe
Targeted Allele Detail

Summary

• Symbol: Wapl^tm1.1Kpfe
• Name: WAPL cohesin release factor; targeted mutation 1.1, Karl Pfeifer
• MGI ID: MGI:7414359
• Synonyms: Wapl^Flox
• Gene: Wapl Location: Chr14:34395885-34469940 bp, + strand Genetic Position: Chr14, 20.84 cM
• Alliance: Wapl^tm1.1Kpfe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331699
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: The targeting vector is designed to insert a loxP site upstream of the endogenous promoter (- 517 bp relative to major transcriptional start site), and a loxP site downstream of exon 2 (+3641 relative to the major transcriptional start site). An FRT-flanked neomycin cassette is also part of the construct. Flp-mediated recombination removed the neo cassette. This allele is a hypomorph, although RNA levels are not altered, WAPL protein levels are reduced by 2 fold. (J:331699)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Wapl Mutation: 169 strains or lines available

References

• Original: J:331699 Kean CM, et al., Decreasing Wapl dosage partially corrects embryonic growth and brain transcriptome phenotypes in Nipbl(+/-) embryos. Sci Adv. 2022 Dec 2;8(48):eadd4136
• All: 1 reference(s)