Wapl^tm1.2Kpfe
Targeted Allele Detail

Summary

• Symbol: Wapl^tm1.2Kpfe
• Name: WAPL cohesin release factor; targeted mutation 1.2, Karl Pfeifer
• MGI ID: MGI:7414361
• Synonyms: Wapl^delta
• Gene: Wapl Location: Chr14:34395885-34469940 bp, + strand Genetic Position: Chr14, 20.84 cM
• Alliance: Wapl^tm1.2Kpfe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331699
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The targeting vector is designed to insert a loxP site upstream of the endogenous promoter (- 517 bp relative to major transcriptional start site), and a loxP site downstream of exon 2 (+3641 relative to the major transcriptional start site). An FRT-flanked neomycin cassette is also part of the construct. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination removed the promoter and exons 1 and 2. (J:331699)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wapl Mutation: 169 strains or lines available

References

• Original: J:331699 Kean CM, et al., Decreasing Wapl dosage partially corrects embryonic growth and brain transcriptome phenotypes in Nipbl(+/-) embryos. Sci Adv. 2022 Dec 2;8(48):eadd4136
• All: 1 reference(s)