Pold4^em1Zzho
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pold4^em1Zzho
• Name: polymerase (DNA-directed), delta 4; endonuclease-mediated mutation 1, Zhongcheng Zhou
• MGI ID: MGI:7423649
• Gene: Pold4 Location: Chr19:4281953-4283688 bp, + strand Genetic Position: Chr19, 3.93 cM
• Alliance: Pold4^em1Zzho page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 744 bp deletion encompassing exon 2 and 3, resulting in a frameshift and theoretically should produce a 52-amino acid mutant protein, however nonsense-mediated mRNA decay should be triggered and result in degradation of the mRNA and thus loss of function. Western blot analysis confirmed absence of protein in various tissues including spleen, lung, liver, kidney, testis and ovary. (J:331883)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pold4 Mutation: 10 strains or lines available

References

• Original: J:331883 Gu X, et al., Pold4 is dispensable for mouse development, DNA replication and DNA repair. Gene. 2023 Jan 30;851:147029
• All: 1 reference(s)