Mphosph8^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mphosph8^{tm1c(EUCOMM)Wtsi}
• Name: M-phase phosphoprotein 8; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7423697
• Synonyms: Mphosph8^fl
• Gene: Mphosph8 Location: Chr14:56905705-56934887 bp, + strand Genetic Position: Chr14, 28.37 cM
• Alliance: Mphosph8^{tm1c(EUCOMM)Wtsi} page
• IMPC: Mphosph8 gene page

Mutation origin

• Mutant Cell Line: EPD0058_2_A03
• Germline Transmission: Earliest citation of germline transmission: J:332344
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 56913526 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 56914365. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:332344)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mphosph8 Mutation: 40 strains or lines available

References

• Original: J:332344 Hagelkruys A, et al., The HUSH complex controls brain architecture and protocadherin fidelity. Sci Adv. 2022 Nov 4;8(44):eabo7247
• All: 1 reference(s)