Adap1^tm1.1Ryya
Targeted Allele Detail

Summary

• Symbol: Adap1^tm1.1Ryya
• Name: ArfGAP with dual PH domains 1; targeted mutation 1.1, Ryohei Yasuda
• MGI ID: MGI:7425227
• Synonyms: CentA1 KO
• Gene: Adap1 Location: Chr5:139257631-139311377 bp, - strand Genetic Position: Chr5, 78.27 cM
• Alliance: Adap1^tm1.1Ryya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:308995
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 (functional domain) was replaced by sequences for LacZ, followed by a translational STOP, and a Neo selection cassette flanked by frt sites. Flp-mediated recombination removed the Neo cassette. The modified locus leads to a fusion protein consisting of exons 1 and 2 and LacZ, while the functional protein is missing. Immunoblot analysis confirmed the complete lack of protein expression in the hippocampus of homozygous mutant mice. (J:308995)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adap1 Mutation: 26 strains or lines available

References

• Original: J:308995 Szatmari EM, et al., ADAP1/Centaurin-alpha1 Negatively Regulates Dendritic Spine Function and Memory Formation in the Hippocampus. eNeuro. 2021 Jan-Feb;8(1):ENEURO.0111-20.2020
• All: 1 reference(s)