Ddx6^em1.2Ysa
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ddx6^em1.2Ysa
• Name: DEAD-box helicase 6; endonuclease-mediated mutation 1.2, Yumiko Saga
• MGI ID: MGI:7425331
• Synonyms: Ddx6^delta
• Gene: Ddx6 Location: Chr9:44516189-44552028 bp, + strand Genetic Position: Chr9, 24.84 cM, cytoband B
• Alliance: Ddx6^em1.2Ysa page

Mutation origin

• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: By using CRISPR/Cas9 technology in ES cells, the exon 5 (ENSMUSE00000216525) was flanked by loxP sequences and was followed by the Pgk-neo cassette flanked by Frt sequences. The Pgk-neo cassette was removed by crossing with Rosa-Flp mice. Cre-mediated recombination removed exon 5. (J:329565)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ddx6 Mutation: 61 strains or lines available

References

• Original: J:329565 Kim J, et al., The RNA helicase DDX6 controls early mouse embryogenesis by repressing aberrant inhibition of BMP signaling through miRNA-mediated gene silencing. PLoS Genet. 2022 Oct;18(10):e1009967
• All: 1 reference(s)