Bloc1s5^em1(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Bloc1s5^em1(IMPC)Tcp
• Name: biogenesis of lysosomal organelles complex-1, subunit 5, muted; endonuclease-mediated mutation 1, The Centre for Phenogenomics
• MGI ID: MGI:7425543
• Gene: Bloc1s5 Location: Chr13:38786674-38821093 bp, - strand Genetic Position: Chr13, 18.35 cM
• Alliance: Bloc1s5^em1(IMPC)Tcp page
• IMPC: Bloc1s5 gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of AAGGGTTAAGTCACACTCCG targeting the 5' side and GTAGTAGGCCATTAACAACT targeting the 3' side of a critical region (ENSMUSE00000291432) along with a single-strand oligonucleotide encoding a Bxb1 attB site. This resulted in a 160-bp deletion of Chr13 from 38,814,930 to 38,815,089 (GRCm39) with insertion of a Bxb1 attB sequence (GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCATACACCGG). (J:265051)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bloc1s5 Mutation: 17 strains or lines available

References

• Original: J:265051 MGI and IMPC, MGI Load of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). Database Release. 2018-2023
• All: 3 reference(s)