Ltbp4^em1(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ltbp4^em1(IMPC)Tcp
• Name: latent transforming growth factor beta binding protein 4; endonuclease-mediated mutation 1, The Centre for Phenogenomics
• MGI ID: MGI:7425804
• Gene: Ltbp4 Location: Chr7:27004561-27037117 bp, - strand Genetic Position: Chr7, 15.87 cM
• Alliance: Ltbp4^em1(IMPC)Tcp page
• IMPC: Ltbp4 gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of ATCTCTGGACCGGTCCAAGC targeting the 5' side and AACCCGCGGTGTTGTCGCAG targeting the 3' side of a critical region (ENSMUSE00001403608, ENSMUSE00001398625, ENSMUSE00001395913 & ENSMUSE00001396773). This resulted in a 2,267-bp deletion of Chr7 from 27,024,452 to 27,026,718 (GRCm39). (J:265051)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ltbp4 Mutation: 120 strains or lines available

References

• Original: J:265051 MGI and IMPC, MGI Load of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). Database Release. 2018-2023
• All: 2 reference(s)