Polr1a^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Polr1a^{tm1c(EUCOMM)Hmgu}
• Name: polymerase (RNA) I polypeptide A; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7432631
• Synonyms: Polr1a^flx
• Gene: Polr1a Location: Chr6:71886037-71956419 bp, + strand Genetic Position: Chr6, 32.21 cM
• Alliance: Polr1a^{tm1c(EUCOMM)Hmgu} page
• IMPC: Polr1a gene page

Mutation origin

• Mutant Cell Line: HEPD0779_7_B03
• Germline Transmission: Earliest citation of germline transmission: J:332795
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 71896885 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 71897931. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:332795)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polr1a Mutation: 94 strains or lines available

References

• Original: J:332795 Falcon KT, et al., Dynamic regulation and requirement for ribosomal RNA transcription during mammalian development. Proc Natl Acad Sci U S A. 2022 Aug 2;119(31):e2116974119
• All: 2 reference(s)