Rimkla^tm1.1Meck
Targeted Allele Detail

Summary

• Symbol: Rimkla^tm1.1Meck
• Name: ribosomal modification protein rimK-like family member A; targeted mutation 1.1, Matthias Eckhardt
• MGI ID: MGI:7432669
• Synonyms: Rimkla^-
• Gene: Rimkla Location: Chr4:119322480-119349795 bp, - strand Genetic Position: Chr4, 55.4 cM
• Alliance: Rimkla^tm1.1Meck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:332696
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRES-Tau (Mapt)-lacZ cassette was introduced immediately downstream of the exon 2 splice acceptor site and exons 2-4 and part of exon 5 were replaced by an FRT-flanked selection cassette consisting of a flp recombinase gene under the control of the testis specific promoter of the angiotensin converting enzyme gene and the neomycin resistance gene. Self-excision of the selection cassette was not seen so it was removed via flp-mediated recombination. A point mutation was identified in the lacZ gene such that beta-galactosidase expression is not observed. Western blot analysis and immunofluorescence in homozygotes confirmed absence of protein in neocortex and hippocampus, and in the retina, respectively. (J:332696)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rimkla Mutation: 21 strains or lines available

References

• Original: J:332696 Becker I, et al., Mice deficient in the NAAG synthetase II gene Rimkla are impaired in a novel object recognition task. J Neurochem. 2021 Jun;157(6):2008-2023
• All: 2 reference(s)