Tg(Crhr2-EGFP/cre)#And
Transgene Detail

Summary

• Symbol: Tg(Crhr2-EGFP/cre)#And
• Name: transgene insertion, David J Anderson
• MGI ID: MGI:7438099
• Synonyms: Crfr2a-eGFPCre
• Transgene: Tg(Crhr2-EGFP/cre)#And Location: unknown
• Alliance: Tg(Crhr2-EGFP/cre)#And page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Recombinase, Reporter)
• Mutation: Insertion
• Tg(Crhr2-EGFP/cre)#And expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Crhr2 (MGI:894312)

• Mutation details: A mouse bacterial artificial chromosome (BAC clone RP23-78P13) containing the entire Crfr2 gene was used to generate the final transgene. With the BAC another gene Inmt was disrupted by inserting a bovine growth hormone polyadenylation sequence (BGHpA) cassette into the 5' end of the Inmt gene that removed the start codon. To simultaneously target Crfr2a and suppress expression Crfr2b from the BAC, a cassette containing an eGFPCre fusion gene followed by an SV40pA was inserted into the 5'UTR of the third exon of the Crfr2 gene, and a STOP codon was introduced into exon IV (shared by both a and b splice forms) to terminate translation of any Crfr2b mRNA transcribed from the BAC. Founder information and line numbers were not available. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:333398)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Crhr2 (mouse) Summary of all recombinase alleles driven by Crhr2. Comparative matrix view of recombinase activities Alliance mouse Crhr2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:333398 Anthony TE, et al., Control of stress-induced persistent anxiety by an extra-amygdala septohypothalamic circuit. Cell. 2014 Jan 30;156(3):522-36
• All: 3 reference(s)