Prdm1^{tm5.1(cre/ERT2)Rob}
Targeted Allele Detail

Summary

• Symbol: Prdm1^{tm5.1(cre/ERT2)Rob}
• Name: PR domain containing 1, with ZNF domain; targeted mutation 5.1, Elizabeth J Robertson
• MGI ID: MGI:7438442
• Synonyms: Prdm1Cre^ERT2, Prdm1Cre^ERT2-LacZ
• Gene: Prdm1 Location: Chr10:44313173-44404497 bp, - strand Genetic Position: Chr10, 23.24 cM
• Alliance: Prdm1^{tm5.1(cre/ERT2)Rob} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:257197
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Mutation: Insertion
• Prdm1^{tm5.1(cre/ERT2)Rob} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Prdm1 (MGI:99655)

• Mutation details: The allele was generated by introducing a cassette containing a CreERT2 upstream of IRES-nlacZ followed by a FRT-flanked neo cassette (CreERT2-IRES-nLacZ-FRT-neo-FRT) into the ATG translational site in exon 3 of the Prdm1 gene via homologous recombination in embryonic stem (ES) cells. Correctly targeted ES cell clones were transiently transfected with a FLP expression construct to excise the neo cassette. (J:257197)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Prdm1 (mouse) Summary of all recombinase alleles driven by Prdm1. Comparative matrix view of recombinase activities Alliance mouse Prdm1 gene page

Expression

In Mice Carrying this Mutation:

10 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prdm1 Mutation: 66 strains or lines available

References

• Original: J:257197 Elias S, et al., Long-lived unipotent Blimp1-positive luminal stem cells drive mammary gland organogenesis throughout adult life. Nat Commun. 2017 Nov 20;8(1):1714
• All: 1 reference(s)